Humanization：
Based on the principle that framework regions (FRs) stabilize the conformation of complementarity-determining regions (CDRs), the AbEngin® Antibody Humanization Platform introduces amino acid mutations at specific sites to optimize the CDR conformation of humanized antibodies. It thereby screens humanized variants whose affinity is comparable to or even superior to that of the parental molecule.Furthermore, all mutated amino acids within the humanization mutation library are of human origin, eliminating the need for backmutation and enabling AbEngin® to deliver a higher degree of humanization.
Technical Advantages：
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Mammalian cell display is a powerful antibody screening tool, which can effectively balance the library capacity and sequence specificity, and ensure the physicochemical properties of antibodies, thereby improving their pharmacability. |
The mammalian display technology can not only be applied in the process of antibody screening, but also play a significant role in antibody engineering. It can be well adapted to humanization (or canine/feline source modification), affinity maturation, and PTM optimization, while maintaining excellent physicochemical properties. |
Mammalian cell display technology is not only applicable to scFv or Fab-based antibodies, but also can efficiently display complex antibody structures such as full-length IgG, VHH, and TCR. It can even directly be used in the screening of antibodies in the form of bispecific antibodies, completing the one-step construction of bispecific antibodies and affinity adjustment in a seamless manner. |
Screening of the full-spectrum configuration：
Mammalian cell display technology VS Other display technologies：
Representative cases 1：Antibody humanization by MCD
Affinity is well maintained or even increased after humanization.
Affinity is further approved by ELISA and Flow cytometry.
| ELISA | Flow cytometry |
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