Technical Principle：
Service Highlights：
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Rapid Antigen PreparationmRNA preparation completed in 1-2 weeks |
Flexible Immunization StrategiesCustom target sequence design |
Longer Expression DurationEfficient Circular/Self-Amplifying mRNA preparation |
More Precise Targeted DeliveryLNP antibody labeling service |
Particle Size Homogeneity Detection of RNA-LNP：
DLS and Cryo-EM data show that the mRNA-LNP prepared by NBLST exhibits excellent particle size uniformity.
Expression Persistence Detection of Different RNA Variants：
Results from in vitro fluorescence assays show that both circRNA and saRNA outperform traditional linear mRNA in terms of gene expression levels and gene expression persistence.
RNA antigens are adopted for immunization based on target analysis. The immunization procedure and timeline are shown below:
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No. |
Time |
Immunization Procedure |
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1 |
Day 0 |
Collect 10 mL of whole blood as reserved negative serum. |
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2 |
Day 1 |
1st: 0.5 mg RNA immunogen; Intramuscular injection at 10 sites. |
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3 |
Day 14 |
2nd: 0.25 mg RNA immunogen; Intramuscular injection at 5 sites. |
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4 |
Day 21 |
Collect 1 mL of whole blood, separate serum, and perform protein ELISA titer testing. |
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5 |
Day 28 |
3rd: 0.25 mg RNA immunogen; Intramuscular injection at 5 sites. |
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6 |
Day 35 |
Collect 1 mL of whole blood, separate serum, and perform protein ELISA titer testing. |
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7 |
Day 42 |
4th: 0.25 mg RNA immunogen; Intramuscular injection at 5 sites. |
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8 |
Day 49 |
Collect 10 mL of whole blood, separate serum, and perform protein ELISA titer testing. Based on titer results:
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